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O objetivo deste trabalho foi avaliar, em cafeeiro suscetível, a proteção contra a cercosporiose, pela aplicação da proteína harpina e acibenzolar-S-metil (ASM), e avaliar seu efeito na germinação de conídios e crescimento micelial in vitro. No primeiro experimento, cafeeiros tratados com ASM (25, 50, 100, 200 &#956;g mL-1) receberam o inóculo de uma suspensão de conídios de Cercospora coffeicola, e a severidade da doença foi avaliada aos 30 e 60 dias após a inoculação. No segundo experimento, cafeeiros foram aspergidos com harpina (7,5, 15, 30, 60, 120 &#956;g mL-1), tendo-se utilizado o mesmo procedimento. No terceiro experimento, plantas aspergidas previamente com ASM (200 &#956;g mL-1) ou harpina (15 &#956;g mL-1) foram tratadas novamente com esses produtos, aos 30 dias após terem recebido inóculo do patógeno. ASM e harpina protegeram os cafeeiros contra cercosporiose 30 dias após a inoculação com C. coffeicola. Entretanto, 60 dias após a inoculação, apenas o ASM (200 &#956;g mL-1), com uma ou duas aplicações, protegeu as plantas contra C. coffeicola. Os cafeeiros foram protegidos contra cercosporiose, em reaplicação de harpina, 30 dias após o primeiro tratamento com essa proteína. Harpina e acibenzolar-S-metil não inibiram o desenvolvimento micelial nem a germinação in vitro dos conídios do patógeno. The objective of this work was to evaluate the protective effect of harpin protein and acibenzolar-S-methyl (ASM) against brown eye spot, in coffee plants, and its effect on in vitro conidial germination and mycelial growth of Cercospora coffeicola. In the first assay, plants treated with ASM (25, 50, 100, 200 &#956;g mL-1) received the inoculum of a C. coffeicola conidial suspension, and the disease severity was evaluated 30 and 60 days after inoculation. In the second assay, plants were sprayed with harpin (7.5, 15, 30, 60, 120 &#956;g mL-1) following the same procedure. In a third trial, plants previously sprayed with ASM (200 &#956;g mL-1) or harpin (15 &#956;g mL-1) were treated again with these products 30 days after pathogen inoculation. ASM and harpin protected the coffe plants against brown eye spot 30 days after inoculation with C. coffeicola. However, 60 days after inoculation, only ASM (200 &#956;g mL-1), with one or two applications, conferred protection to plants against C. coffeicola. Coffee plants were protected against cercosporiosis, when harpin was reapplied on plants 30 days after a previous treatment with this protein. Harpin and acibenzolar-S-methyl did not inhibit the in vitro conidial germination and the mycelial growth of the pathogen

Proteção do cafeeiro contra cercosporiose por acibenzolar-S-metil e proteína harpina