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The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months. O objetivo deste trabalho foi avaliar a influência de diferentes substratos e pré-tratamentos in vitro, na aclimatização de plantas in vitro de Arachis retusa. As plantas foram transferidas para Plantmax ou areia e adubadas com solução de Hoagland. Plantas mantidas em areia, com adubação ou sem adubação, apresentaram maiores taxas de sobrevivência. Para avaliação da influência de pré-tratamentos in vitro, segmentos de caule foram cultivados em meio MS suplementado com diferentes concentrações de sacarose. As maiores taxas de sobrevivência e desenvolvimento foram observadas em plantas cultivadas em sacarose a 1,5% e 3%. Depois de cinco meses, foram observadas a floração e a produção de frutos

Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa