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O objetivo deste trabalho foi avaliar a atividade da amilase no quimo da rã-touro, Rana catesbeiana Shaw 1802 (3,6 a 200 g). Oitenta e sete animais foram distribuídos em baias-testes com temperatura e fotoperíodo controlados. As rãs selecionadas na fase pós-metamórfica receberam ração comercial extrusada ad libitum. Durante 87 dias de experimento, foram efetuadas 29 coletas em intervalos de 1 a 8 dias. As coletas do conteúdo intestinal foram feitas mediante a insensibilização das rãs em gelo e água e isolamento posterior do intestino delgado das mesmas. Para os testes da atividade da amilase, foram utilizados kits enzimáticos comerciais. A atividade da amilase foi observada a partir do terceiro dia e apresentou aumento até alcançar estabilidade, quando os animais pesavam 4,35 g. Do sétimo dia até o final do experimento, ocorreu a manutenção da estabilidade da atividade (137,44 UI) e atividade específica da amilase (14.688 UI mg-1). The objective of this work was to evaluate the amylase activity in the chime of the bullfrog Rana catesbeiana Shaw 1802 (3.6 to 200 g). Eighty-seven animals were distributed in test stalls with controlled temperature and photoperiod. The frogs selected in the post-metamorphic phase received commercial extruded diet ad libitum. During 87 days of the experiment, 29 samples were collected in intervals varying from one to eight days. The intestinal content samples were made by first desensitizing of the frogs in ice and water, and subsequently removing the small intestine. For the tests of amylase activity, commercial enzymatic kits were used. Amylase activity was observed since the third day and it increased until reaching stability, when the animals weighed 4.35 g. Amylase activity (137.44 UI) and specific activity (14,688 UI mg-1) remained stable from the seventh day until the end of the experiment

Atividade da amilase em rã-touro durante a fase pós-metamórfica