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Drosophila piratininga sp. nov. and Drosophila sampa sp. nov., belonging to the canalinea group of the subgenus Drosophila, are described based mostly on wild-caught specimens collected at a forest reserve of the Cidade Universitária "Armando de Salles Oliveira", an urban remnant of the montane Atlantic Forest located at west São Paulo city, state of São Paulo, Brazil. The two species are readily distinguished from each other mostly by having different wing patterns: the main crossveins being remarkably clouded in the first one and unclouded in the latter. Illustrations of terminalia are also provided. Drosophila piratininga sp. nov. e Drosophila sampa sp. nov., espécies neotropicais pertencentes ao grupo canalinea, são descritas no presente artigo. Elas podem ser facilmente separadas com base no padrão das asas, sendo que as duas nervuras transversais são nitidamente esfumaçadas na primeira e hialinas na segunda. As coletas foram realizadas na Reserva Florestal da Cidade Universitária "Armando de Salles Oliveira", um remanescente da Mata Atlântica de planalto localizado na área urbana da zona oeste do município de São Paulo, Estado de São Paulo, Brasil. Ilustrações detalhadas das terminálias são fornecidas

Two new species of Drosophila belonging to the canalinea group (Diptera, rosophilidae)