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A new species, Isoctenus malabaris, is described from southern Brazil. This spider was abundantly collected with pitfall traps at Araucaria Forests (Mixed Ombrophilous Forest) domain. The activity of this species was studied in three distinct habitats (primary and secondary forests and silvicultures) during 20 months. A bimodal seasonal activity pattern, of males, was observed. Abundance differences of this species between habitats were not significant. Uma nova espécie, Isoctenus malabaris, é descrita para sul do Brasil. Espécimes desta aranha foram abundantemente coletados com armadilhas de queda em área de domínio da Floresta com Araucária (Floresta Ombrófila Mista). A atividade da espécie foi estudada em três hábitats distintos (florestas primárias e secundárias e silviculturas) ao longo 20 meses. Foi observado um padrão bimodal de atividades ao longo do ano, para espécimes machos. A diferença na abundância desta espécie entre os hábitats não foi significativa

Description and ecological notes on Isoctenus malabaris sp. nov. (Araneae, Ctenidae) from southern Brazil