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The emergence of new respiratory viruses including: the Severe Acute Respiratory Syndrome; the coronavirus in 2003 (SARS-CoV); influenza A(H1N1)pdm09, in the human population in the past ten years; the emergence of a new coronavirus identified in a patient presenting severe acute respiratory syndrome who travelled to Saudi Arabia (June, 2012), denominated as Middle East Respiratory Syndrome (MERS-CoV); and laboratory confirmation of three human infections with an avian influenza A(H7N9) virus not previously reported in humans on March 29, 2013, has encouraged scientists to develop diagnostic tests to identify these viruses. The Adolfo Lutz, a Public Health Institute, has been improving its methodology tools in order to provide a timely answer to public health authorities facing acute respiratory disease outbreaks.

DIFFERENTIAL DIAGNOSIS OF RESPIRATORY VIRUSES BY USING REAL TIME RT-PCR METHODOLOGY