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Heterodera glycines is a phytonematode of economic importance in the soybean crop. Therefore, the objective was to compare methodologies for evaluation of soybean genotypes in relation to H. glycines. Two experiments were conducted (trials A and B) under greenhouse condition in a completely randomized design with six replications. In the trial A, ceramic pots of 0.8 dm3 and standard methodologies of inoculation and assessment were used. In the trial B, polyethylene pots filled with 0.5 dm3 infested soil and visual analysis were used. Promising genotypes obtained by different soybean genetic breeding programs were evaluated. Seventy four percent of the studied genotypes presented similar numbers of females in both trials. The correlation coefficient between the number of female in trial A and the number of female in trial B was 0.85 (p<0.01) and between the number of female in trial A and the visual rating of the number of females in trial B was 0.90 (p<0.01). The standard methodologies of inoculation and evaluation using infested soil and visual analysis were, in general, mutually consistent. Genotypes identified as resistant (UFVS 2010 and BCR1070G229) and moderately resistant (BCR132390, BCR945G114 and BCR1057G163) to the cyst nematode race 3 were found by the two methods of assessment.

Avaliação de genótipos de soja em relação ao nematoide de cisto