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Visando desenvolver metodologia para cultura de meristemas de videira in vitro foram testadas quatro doses do hormônio 6-BA (6-benzilaminopurina) em meio primário, o qual constou do meio básico de Nitsch & Nitsch. Brotos apicais do cultivar A Dona foram esterilizados, os meristemas com um ou dois primórdios foliares foram excisados e, após breve período de pré-incubação, inoculados em meio primário. Os explantes foram avaliados quanto a viabilidade, tamanho, número de folhas e rapidez no desenvolvimento, concluindo-se que as duas doses intermediárias, quais sejam, 2,0 e 5,0µM de 6-BA foram as mais apropriadas para utilização em meio primário para o cultivar A Dona. Aimming at the development of a metodology for 'in vitro' grapevine meristem tip culture, the effects of four concentrations (1.0; 2.0; 5.0 and 10.0µM) of 6-BA (6 benzylaminopurine) cytokinin on growth and development of grapevine 'A Dona' isolated meristem tips in primary medium using basal medium of Nitsch & Nitsch were tested. Shoot tips were esterilized, meristems were then excised and after a brief period of pre-incubation were inoculated ira primary mediam. Viability, size, number of leaves and development speed of those meristem tips were studied. The concentrations of 2.0 and 5.0µM seemed to be the most appropriate to seedless A Dona cultivar

Cultura in vitro de meristemas de videira. I: concentrações do hormônio 6-BA em meio primário