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Partículas esferoidais de 50-60 m¼ de diâmetro foram encontradas em preparações rápidas e em secções ultra-finas de fôlhas de mandioca infetadas pelo vírus do mosaico das nervuras (VMNM), examinadas ao microscópio electrônico. Tais partículas não foram observadas em preparações de plantas sadias ou de fôlhas sem sintomas de plantas infetadas, e são consideradas como representando o VMNM. Em secções ultra-finas de tecido foliar, as partículas do VMNM encontravam-se dispersas em certas áreas do citoplasma, ricas em ribosomas mas pobres em outras organelas citoplasmáticas, e onde esporadicamente apareciam pequenos feixes de fibrilas densas (7-10 m¼ X 100-300 m¼). Massas densas, de textura finamente granulada (0,3-3 ¼ de diâmetro), foram encontradas em alguns casos associadas às partículas. Provàvelmente, tanto as massas densas como as fibrilas estão implicadas na síntese do VMNM. Spheroidal particles, 50-60 m¼ in diameter, were found in quick preparations and in ultra-thin sections for electron microscopy, from leaves of cassava plants infected with the vein mosaic (CVMV). They were not found in preparations made from healthy plants, nor in symptomless leaves of infected plants, and are considered as representing the virus. In ultra-thin sections of leaf tissues, CVMV particles were found dispersed in certain areas of the cytoplasm, rich in ribosomes, but poor in other cytoplasmic organelles, where sporadical small bundles of dense fibrils (7-10 m¼X 100-300 m¼) occur. Dense and fine granular masses (0.3-3 ¼,in diameter) were seen in a few instances, associated with the particles. It seems likely that both fibrils and the dense masses are primarily involved in the CVMV synthesis

Partículas esferoidais associadas ao vírus do mosaico das nervuras da mandioca