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Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum. A identificação bioquímica de micobactérias não permite a discriminação das espécies do complexo Mycobacterium avium (MAC). Este estudo mostrou que a amplificação por PCR de DT1 e DT6, duas sequências de cópia única identificadas no genoma de M. avium sorotipo 2, da sequência de inserção IS1245, encontrada consistentemente em cepas de M. avium e de um fragmento do gene da proteína de choque térmico hsp65, seguida da análise do polimorfismo de restrição, são testes rápidos e acurados para a diferenciação das espécies M. avium, M. intracellulare e M. scrofulaceum

Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction