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The sea cucumber Acaudina leucoprocta H. L. Clark (EchinodermataI: HolothuroideaI: MolpadidaI: Caudinidae) is one of the most common sea products in Asia (Liu et al., 2017, 2018). Ot possesses a purple-brown and spindle-shaped body, generally 10-14 cm long, with a white posterior and cloacal lining (Clark, 1938). Ot contains a variety of active components and has high nutritional and pharmaceutical values comparable to Stichopus japonicas (Shan et al., 2013). However, due to long living in the submarine silt, its body wall is covered by dense and stiff dirt, in which seabed silt and mucus secreted from the surface of A. leucoprocta were strongly linked, influencing the food value of A. leucoprocta (Shan et al., 2013). Additionally, rapid developments of industrialization and urbanization have generated severe marine pollution, causing increased contents of toxic heavy metals (As, Hg, Pb, and Cd) in the surface of A. leucoprocta (Lin et al., 2018). The consumption of A. leucoprocta has been drastically hampered (Huang et al., 2007). Up to a million tons of A. leucoprocta have been harvested from the East China Sea and discarded as waste by the local fishermen for many years (Lin et al., 2017). Therefore, optimized utilization of these polluted A. leucoprocta is necessary, especially for preparation of the valuable products.

Optimization of extraction process for efficient imino acids recovery and purification from low-value sea cucumber