English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

1. Received: Jun. 25, 2018. Accepted: Sep. 10, 2018. Published: Mar. 28, 2019. DOI: 10.1590/1983-40632019v4953673. 2. Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanidade, Curitiba, PR, Brasil. E-mail/ORCID: anapauladeazevedo@gmail.com/0000-0002-1430-6495, biasi@ufpr.br/0000-0002-3479-8925. 3. Universidade Federal do Paraná, Departamento de Patologia e Parasitologia, Curitiba, PR, Brasil. E-mail/ORCID: gabrielaxavier13@gmail.com/0000-0002-5644-5653. 4. Universidade Federal do Paraná, Departamento de Botânica, Curitiba, PR, Brasil. E-mail/ORCID: hugofraga@ufpr.br/0000-0002-7363-5213, mquoirin@ufpr.br/0000-0003-3190-6895. In plant micropropagation, the establishment stage is difficult, due to the presence of microorganisms in tissues from field-grown matrices, especially for bamboo. This study aimed to establish an efficient asepsis protocol for Bambusa oldhamii explants from field plants, as well as to carry out the molecular identification of a possible endophytic bacterial isolate. The explants were exposed to 70 % alcohol, 1 % sodium hypochlorite (NaOCl), 0.1 % mercuric chloride (HgCl2), thiophanate-methyl (Cercobin®) and chlorhexidine digluconate (2 % Riohex®) in different combinations, and introduced into Murashige and Skoog culture medium (solid or liquid), supplemented or not with 4 mL L-1 of Plant Preservative Mixture (PPMTM), totaling seven treatments. The asepsis and immersion of the explants in the liquid culture medium containing 4 mL L-1 of PPMTM visually inhibited the bacterial and fungal growth, allowed the development of shoots with a mean length of 2.2 cm and posterior subcultures, being the best treatment used for the in vitro establishment of B. oldhamii. The molecular identification of an endophytic bacterium performed by 16S rDNA sequencing allowed to identify the bacterial isolate as Ralstonia sp., with 100 % of similarity, and the phylogenetic analysis grouped it with Ralstonia pickettii. In addition, the bacterial isolate showed to be sensitive to 4 mL L-1 of PPMTM by the minimum inhibitory concentration test.

In vitro establishment of Bambusa oldhamii Munro from field-grown matrices and molecular identification of endophytic bactéria