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The aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary Imunomarcação de metaloproteinases 9 (MMP-9) e 2 (MMP-2) e seus inibidores (TIMP-1) e (TIMP-2) no tecido mamário canino e neoplásico Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue Hugo Henrique Ferreira1 , Denise Caroline Toledo1 , Adriana Marques Faria1 , Diego Pereira Araújo1 , Andrigo Barboza di Nardi2 , Regiani Nascimento Gagno Porto1 , Veridiana Maria Brianezi Dignani de Moura1 1Universidade Federal de Goiás (UFG), Goiânia, GO, Brasil 2Universidade Estadual Paulista, Jaboticabal (UNESP), SP, Brasil *Correspondente hugo.hen@hotmail.com