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The limiting factor in the cryopreservation process of\udequine semen is related to the species, as they present a\udgreat variability in the ejaculate’s characteristics after\udthawing. The aim of this study was to evaluate sperm\udviability after thawing, from Quarter Horse and\udMangalarga Marchador stallions using two extenders\ud(Botu-crio and Tris) in cryopreservation. To this end, we\udanalyzed the physical characteristics of fresh semen, the\udsperm membrane functionality by the hypoosmotic\udswelling test (HOST), total motility and vigor by the\udThermoresistance Test (TRT), progressive motility by a\udcomputerized system CASA (Computer-Assisted Semen\udAnlyses) and acrosomal membrane integrity and\udfunctionality of the mitochondria by epifluorescence\udmicroscopy. After thawing, the extender Botu-crio®\udbetter preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between\udbreeds for the thermoresistance test after twaing. Quarter\udHorse showed higher percentage of bigger defects in the\udsperm pathology analysis

Eficiência dos diluidores Tris e Botu-crio® sobre os parâmetros seminais de garanhões das raças quarto de milha e mangalarga marchador