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The objective of the study was to investigate the influence of anise seed (Pimpinella anisum L.) powder supplemented to the drinking water on selected blood parameters of broilers. In total, 360 one-day-old Hubbard Classic chicks were randomly divided into four groups with three replicates of 30 birds each. Anise seed powder was added at 0, 500, 750, and 1000mg/L to the drinking water offered to the C (control), T1, T2, and T3 groups respectively. The supplement was supplied for 56 days. When broilers were 28 and 56 days old, blood samples were collected (30 birds per group) by brachial vein puncture to evaluate the cellular components of blood (RBC, WBC, Hgb, HCT, H/L). The following serum biochemical parameters were evaluated: CHOL, GLU, TP, albumin, globulins, Ca, P, TG, total lipids, UA, and creatinine, as well as AST and ALT enzyme activities. The statistical analysis indicated that the anise supplement significantly improved blood RBC, WBC, Hgb, HCT, TP, albumin, globulin, GLU, P, and Ca levels of broilers in groups T4, T3, and T2 compared with the control group on days 28 and 56, and on average. Also, T3, T2 and T1 presented lower H/L ratio and CHOL, TG, total lipids, creatinine, UA, AST and ALT serum levels compared with the control group. The highest inclusion levels of anise seed powder, i.e., 1000 and 750mg/L, had a stimulating effect on the physiological traits of the birds

brazilian journal of poultry science

Effect of Various Concentrations of an Anise Seed Powder (Pimpinella Anisum L.) Supplement on Selected Hematological and Biochemical Parameters of Broiler Chickens