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The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls.    Material and methods   The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of  Streptococcus mutans  (BT) and the direct contact test (DCT) by exposure to  Enterococcus faecalis  for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05).    Results   Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest  S. mutans  biofilm accumulation (p=0.023). Only the Group L showed continued effect against  E. faecalis  after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups.    Conclusions   These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against  S. mutans , while Luxatemp showed  in vitro  inhibition of  S. mutans  biofilm accumulation and  E. faecalis  growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic.

Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials