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In the article Hubacek et al. (2014) published in this journal, a genetic variant of CHRNA3 was examined. We think that molecular sizes for the PCR product and the restriction fragments for the analysis of rs578776 were presented erroneously in Table 1. In this table, it said that the PCR product encompasses 167 base pairs (bp) and the restriction fragment sizes are 100 bp and 67 bp for the C allele when the PCR product was cut by the enzyme NlaIII. However, the actual size of the PCR product should be 146 bp, and restriction fragments should be 82 bp, 54 bp and 10 bp for the C allele; and 92 bp and 54 bp for the T allele. In Figure 1 we describe here the PCR product and the restriction fragments predicted by using the University of California Santa Cruz (UCSC) Genome Browser In Silico PCR Tool with the primers 5’-TTC TTT ACT GGG TCT AAA GGG CTA TGC C-3’, 5’-ATC CAC CCA GTT TAT GGT GTA CTA AG-3’ and the restriction enzyme NlaIII, as used in Table 1 of the article. We also performed an in silico restriction analysis using NEBcutter V2.0. Figure 2 demonstrates an actual example of the restriction pattern obtained in our laboratory.

genetics and molecular biology

Letter to the Editor regarding Hubacek et al.’s report “Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study”