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The present study evaluated the effect of feed particle size, thermal processing different levels of fat inclusion and of moisture addition on the amount of gelatinized starch in a corn-soybean broiler diet. The different processing factors were combined in a 2 x 4 x 4 x 2 factorial arrangement in a three randomized block design consisting of three production series: two particle sizes (coarse: 1041 microns and medium: 743 microns), four fat inclusion levels at the mixer (15, 25, 35, and 45 g/kg of feed), four moisture addition levels in the conditioner (0, 7, 14, and 21g/kg of feed), and two thermal processing treatments (conditioning-pelleting or conditioning-expanding-pelleting) which resulted in 64 different processed feeds. For the determination of the amount of gelatinized starch one feed sample was collected per treatment in each of three production series, totaling three replicates/treatment. Data were transformed using a variation of Box-Cox transformation in order to fit normal distribution (p>0.05). Adding moisture up to 21g/kg of feed in the conditioner linearly increased the amount of gelatinized starch (p<0.05). The conditioner-expander-pelleting treatment of the diets (at 110°C) increased (p<0.05) the degree of starch gelatinization from 32.0 to 35.3 % compared with the conditioner-pelleting treatment (at 80-82°C). The gelatinized starch content increased from 30.2 to 37.2% in the feed (p<0.05) as the particle size increased from medium to coarse. Fat inclusion had a quadratic effect (p<0.05) on starch gelatinization. The degree of starch gelatinization was significantly reduced with fat inclusion levels higher than 35 g/kg of diet. The factors evaluated in this study resulted in interactions and significant effects on degree of starch gelatinization

Impact of particle size, thermal processing, fat inclusion, and moisture addition on starch gelatinization of broiler feeds