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Several long non-coding RNA (lncRNA) might be correlated with the prognosis of colorectal cancer (CRC) and serve as a diagnostic and prognostic biomarker. However, the exact expression pattern of small nucleolar RNA host gene 12 ( SNHG12 ) in colorectal cancer and its clinical significance remains unclear. The level of  SNHG12  was detected by qRT-PCR in CRC tissues and CRC cells. MTT assay and colony formation assay were performed to examine the cell proliferation of CRC cells transfected with pcDNA- SNHG12  or si- SNHG12 . Flow cytometry technology was used to detect cell cycle and cell apoptosis of CRC cells transfected with pcDNA- SNHG12  or si- SNHG12 . The protein level of cell cycle progression-related molecules, including cyclin-dependent kinases (CDK4, CDK6), cyclin D1 (CCND1) and cell apoptosis-related molecule caspase 3 was detected by western blot. The effect of  SNHG12  knockdown was examined  in vivo . Increased levels of  SNHG12  were observed in CRC tissues and in CRC cells.  SNHG12  promoted the cell proliferation of CRC cells. In addition,  SNHG12  overexpression boosted the cell cycle progression of SW480 cells transfected with pcDNA- SNHG12  and  SNHG12  knockdown inhibited the cell cycle progression of HT29 cells transfected with si- SNHG12 .  SNHG12  also inhibited the cell apoptosis of CRC cells. We also found that  SNHG12  increased the expression of cell cycle-related proteins and suppressed the expression of caspase 3. Our results suggest that  SNHG12  promoted cell growth and inhibited cell apoptosis in CRC cells, indicating that  SNHG12  might be a useful biomarker for colorectal cancer.

LncRNA SNHG12 promotes cell growth and inhibits cell apoptosis in colorectal cancer cells