English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This manuscript presents evaluation of various strategies used for the scale-up of chromatographic purification systems. Towards that end, the manuscript provides development and optimization of a single-step whey protein concentrate (WPC) fractionation process at lab scale on a 1.0 × 19 cm column using UNOsphere S. Subsequently, scale-up of this newly developed process and conduction of trials at higher scale is also undertaken by applying various scale-up strategies. For these trials, this work categorized various scale-up factors into constant velocity (CV) and constant bed volume per hour (CBVH) scale-up methods. Scale-up trials in bigger diameter (1.5 cm and 2.5 cm) columns with height to diameter ratio (H/D) =19 and <19 were performed. The effects of changing flow velocity and H/D ratio due to the applied scale-up strategy were evaluated and compared with lab performance in terms of resolution vs. lower equivalent to theoretical plate (HETP) relationship. The CBVH method was found to be an efficient method for the scale-up of the developed whey protein resolution system. Also an equation for the prediction of resolution in a scaled column on the basis of a HETP-resolution correlation is presented. It is useful for the prediction of either resolution or bed height in scaled column of WPC or similar biomolecular purification systems.

brazilian journal of chemical engineering/brazilian journal of chemical engineering

DEVELOPMENT AND OPTIMIZATION OF A SINGLE-STEP CATION CHROMATOGRAPHIC WHEY PROTEIN FRACTIONATION PROCESS: EVALUATION AND COMPARISON OF SCALE-UP STRATEGIES