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portuguesA mandioca (Manihot esculenta Crantz) e uma importante cultura para o Brasil, onde o Para e o principal produtor de raizes. Regioes tropicais com alta umidade e temperatura favorecem o desenvolvimento de diversas doencas, como as podridoes de raiz. O presente estudo tem como objetivo avaliar o efeito da luminosidade e de meios de cultura no crescimento micelial e na esporulacao de Phytopythium sp. e analisar metodos de inoculacao do patogeno em raizes de mandioca destacadas. Os ensaios in vitro foram instalados em esquema fatorial 2x6 (luminosidade x meio de cultura), com cinco repeticoes e as medias comparadas pelo teste t (p≤0,05). O meio de cultura contendo raiz de mandioca mansa proporcionou maior desenvolvimento micelial e maior esporulacao do patogeno e e o mais adequado para o cultivo do patogeno. O cultivo sob ausencia de luz gerou melhor crescimento micelial do que o cultivo sob 12 horas de luz. Quanto ao tipo de inoculacao, a resposta foi melhor nas raizes que obtiveram ferimentos mais profundos. EnglishCassava (Manihot esculenta Crantz) is an important crop in Brazil and Para is the major producer of roots. High temperature and humidity of tropical regions favor the development of various diseases, among them the cassava root rot. The objective of this study was to evaluate the effect of luminosity and culture medium on the mycelial growth and sporulation of Phytopythium sp. associated with different methods of inoculation on cassava roots. In vitro tests for pathogen growth were established in a 2 x 6 factorial design (luminosity x culture medium) with five replicates and the means were compared by t test (P≤0.05). The culture medium containing sweet cassava root produced greater mycelial development and higher pathogen sporulation and it was the most suitable medium for pathogen culture. The culture under absence of light generated better mycelial growth than culture under 12 hour of light. Regarding the type of inoculation, the response was better when deeper injuries were induced.

Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.