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EnglishIn this work, we describe an unusual case of fibrinous pleuropneumonia caused by Pasteurella multocida associated with generalized lymphadenomegaly in a bovine. The animal had a one-month history of generalized superficial lymphadenomegaly that progressed to anorexia and submandibular oedema, resulting in spontaneous death. At necropsy, the parenchyma of the lymph nodes and multiple organs was obliterated by a dense proliferation of round neoplastic cells (lymphoma). Additionally, the neoplasm presented multifocal areas of haemorrhage and necrosis, characteristic of lymphoma. The parietal and visceral pleura and parietal pericardium were enlarged and covered diffusely with large amounts of a yellowish fibrillary material. The lungs were mildly enlarged, non-collapsed, and firm and exhibited interlobular septae that were thickened with a gelatinous material. Histopathological examination showed that the parietal and visceral pleura were enlarged due to a diffuse and severe inflammatory infiltrate composed of degenerate neutrophils associated with severe fibrin deposition, characteristic of fibrinous pleuropneumonia. Pleura and parietal pericardium fragments were cultivated in aerobic and microaerobic microbiological conditions. Round greyish colonies of gram-negative coccobacilli that were shiny and non-haemolytic were observed in sheep blood agar. The biochemical profile was indicative of Pasteurella spp. Molecular identification was performed by partial 16S rRNA amplification following sequencing. Pasteurella multocida was confirmed as the primary bacterium associated with the bovine fibrinous pleuropneumonia. We are able to infer that the lymphoma caused immunodepression, which increased the animal’s susceptibility to atypical infectious microorganisms such as pathogenic P. multocida. Key words: P. multocida; bovine pasteurellosis; immunodepression; enzootic bovine leukosis; molecular identification. portuguesNesse trabalho, relatamos um caso de pleuropneumonia fibrinossupurativa causada por Pasteurella multocida associada a linfoadenomegalia em um bovino. O animal apresentava aumento generalizado de linfonodos ha um mes progredindo para anorexia e edema submandibular por tres dias culminando com obito. Durante a necropsia, tanto dos linfonodos quanto de diversos orgaos evidenciaram proliferacao neoplasica de celulas arredondadas e arranjadas em mantos (linfoma). Adicionalmente, areas multifocais de hemorragia e necrose, caracteristicas de linfoma, foram observadas. As pleuras parietal e visceral e pericardio parietal apresentavam-se espessas e recobertas por acentuada quantidade de fibrina. Os pulmoes estavam aumentados, nao colabados, firmes e exibiam espessamento com edema moderado de septos interlobulares. A microscopia, cortes da pleura visceral exibiram acentuado infiltrado inflamatorio de neutrofilos degenerados com intensa deposicao de fibrina, caracteristicas da pleuropneumonia fibrinossupurativa, alem de neovascularizacao e proliferacao de fibroblastos. Amostras de pulmao e da pleura foram cultivadas em aerobiose e microaerobiose. Evidenciou-se o crescimento puro no agar sangue ovino de colonias redondas, acinzentadas, brilhantes e nao-hemoliticas, sendo caracterizadas como cocobacilos gram-negativos. As caracteristicas bioquimicas do isolado foram condizentes com Pasteurella spp. Procedeu-se a identificacao molecular do isolado atraves da amplificacao parcial do gene rRNA 16S com posterior sequenciamento do produto amplificado. Deste modo foi possivel a confirmacao do isolado como Pasteurella multocida, sendo o agente primario da pleuropneumonia fibrinosa. Com estes dados, podemos afirmar que o linfoma causou um quadro de imunodepressao, a qual aumenta a susceptibilidade dos animais a agentes infecciosos atipicos, como a P. multocida patogenica. Palavras-chave: P. multocida; pasteurelose bovina; imunodepressao; leucose enzootica bovina; identificacao molecular.

Fibrinous pleuropneumonia caused by Pasteurella multocida associated with bovine lymphoma