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EnglishTo know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter 1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes. Key words: fish reproduction; fish oocytes; toxicity; concentrations of cryoprotecntants; Steindachneridion parahybae portuguesConhecer os crioprotetores nao toxicos aos oocitos de peixes e de extrema importância para testes que visam aumentar a resistencia dos oocitos ao frio, permitindo, assim, estudos mais avancados em criopreservacao. Desta forma, crioprotetores comumente utilizados como o metanol, dimetil sulfoxido, etilenoglicol, propilenoglicol, sacarose e frutose foram estudados. Os oocitos imaturos, nos estagios inicial ate vitelogenico (diâmetro 1,8mm) de Steindachneridion parahybae foram avaliados. Quatro experimentos distintos foram realizados, sendo tres destes utilizando oocitos imaturos, e um usando oocitos no estagio maduro. Para cada estagio oocitarios foram avaliados, considerando qual a melhor solucao de manutencao a ser utilizada: Hank ou 50% L15 e; viabilidade apos banhos por 30min (temperatura ambiente) em concentracoes de crioprotetores, variando de 0,25 a 4M. Diferentes testes foram utilizados para avaliar a viabilidade dos oocitos: maturacao in vitro seguido por observacao da quebra da vesicula germinativa (somente para oocitos imaturos), coloracao por Azul de Tripan (todos os estagios) e taxas de fertilizacao e eclosao (somente no estagio maduro). Os resultados mostraram que o efeito toxico dos crioprotetores em oocitos geralmente crescem com o aumento das concentracoes. A sensibilidade dos oocitos a crioprotetores aumentam de acordo com o estagio de desenvolvimento, com oocitos maduros sendo mais sensiveis. Sacarose, frutose, metanol, propileno glicol e dimetil sulfoxido podem ser usados como crioprotetores para oocitos de S. parahybae. Palavras-chave: reproducao de peixe; oocitos de peixes; toxicidade; concentracoes de crioprotetores; Steindachneridion parahybae

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants