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EnglishCochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P Key words: autoclaving; cerrado biome; active chlorine; in vitro cultivation portuguesAs raizes de Cochlospermum regium sao usadas na medicina popular, pois seu extrato apresenta diversas moleculas fitoquimicas, algumas com atividade antimicrobiana, que, consequentemente, expoe esta especie ao risco de erosao genetica. Assim, o objetivo deste estudo foi elaborar um protocolo de multiplicacao in vitro para explantes de C. regium usando esterilizacao quimica do meio de cultura. Gemas apicais obtidas de plântulas de C. regium germinadas in vitro foram inoculadas em meio de cultivo MS suplementado com 0,05mg L-1 de ANA e 1mg L-1 de BAP e esterilizado pela adicao do agente quimico hipoclorito de sodio (NaOCl) nas concentracoes de cloro ativo de 0,001%, 0,003% e 0,005%. O meio autoclavado foi utilizado como controle. Os resultados mostraram que a contaminacao por bacterias aos 91 dias de cultivo foi significativamente (P Palavras-chave: autoclavagem; bioma cerrado; cloro ativo; cultivo in vitro

Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium