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Trifoliate orange is one of the rootstocks most used worldwide mainly because it provides excellent quality fruit production. Harvest at the correct time is among factors that determine the physiological quality of seeds. The aim of this study was to identify physicochemical changes in ‘Limeira-IAC382’ trifoliate orange fruits, searching for the ideal harvest moment to obtain high quality seeds. Fruits were harvested at 180, 210 and 240 days after anthesis, stored in cold chamber and evaluated at 0, 15, 30 and 45 days of storage. The following variables were determined: mass loss, fruit color index, soluble solids, total titratable acidity and fruit juice ratio. Seeds were manually extracted and dried until reaching 30% water content. Seeds were used for the performance of germination, electrical conductivity and greenhouse seedling emergence tests. Seeds from fruits harvested at 240 DAA showed higher germination and greenhouse seedling emergence percentage compared to the other treatments until 30 and 45 days of storage in cold chamber, respectively. Storage for 45 days in cold chamber of fruits harvested at 210 DAA increases seed germination rate and the percentage of seedlings from seeds harvested at 180 DAA. It could be concluded that the harvesting of ‘Limeira-IAC382’ trifoliate orange fruits should be performed when fruits reached yellowish peel and low juice ratio, aiming at obtaining seeds with physiological quality.

Ideal harvesting point of ‘Limeira-IAC382’ trifoliate orange fruits for seed extraction