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BACKGROUND    Nyssorhynchus deaneorum  is a potential malaria vector because it has been shown to be competent to transmit  Plasmodium vivax  and  Plasmodium falciparum , and because it exhibits antropophilic and endophilic behaviors in some regions of the Amazon. This profile makes  Ny. deaneorum  a useful mosquito for experiments that model  Plasmodium- vector interactions in the Amazon.    OBJECTIVE   Herein we describe how a free-mating colony of  Ny. deaneorum  has been established using an automated light stimulation system.    METHODS   Mosquitoes were captured in São Francisco do Guaporé, Rondônia. The F1 generation was reared until adult emergence at which point copulation was induced using an automatic copulation induction system (ACIS).    FINDINGS   After four generations, natural mating and oviposition began to occur without light stimulation. The number of pupae and adult mosquitoes increased from the F 5  to F 10  generations. The new  Ny. deaneorum  colony exhibited susceptibility to  P. vivax.MAIN CONCLUSIONS   Automated light stimulation is an effective method for establishing an  Ny. deaneorum  colony under laboratory conditions as it produces enough adults to create a stenogamic colony. The establishment of a stable,  P. vivax -susceptible colony of  Ny. deaneorum  makes it possible to model parasite-vector interactions and to test novel drug therapies that target parasite development in mosquitoes.

Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil