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Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify  Leptospira  isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species.METHODS:   We used comparative genomic analysis between 35  Leptospira  species to find new molecular targets for  Leptospira  species identification.RESULTS:   We proposed the use of the  rpoC  gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35  Leptospira  species.CONCLUSIONS:   The  rpoC  gene can be a molecular target to identify the main species of the  Leptospira  genus directly from clinical samples.

New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA