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Previous studies that detected  Paracoccidioides  spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of  Paracoccidioides  spp. DNA in soil samples from an urban area of southern Brazil.METHODS:   Sixteen soil samples were submitted to nested-PCR and the amplicons of a representative number of positive samples were sequenced.RESULTS:    Paracoccidioides  spp. DNA was found in 44% of samples. Four DNA amplicons were sequenced, showing 100% homology with  P. brasiliensis .CONCLUSIONS:   The southern Brazilian urban population is commonly exposed to the  Paracoccidioides  fungus.

Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil