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Â  Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with 80% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A 260 /A 280 ratio ranged from 1.81 to 1.99 and A 260 /A 230 ratio was over 2.0), and high in yield.

Evaluation of Six Methods for Extraction of Total RNA from Loquat