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A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 I¼M BA. Callus growth was observed on MS medium containing 44.3 I¼M BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 I¼M), NAA (5.3, 16.1, 26.8 I¼M) or BA (4.4, 13.3, 22.1 I¼M) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 I¼M BA. All regenerated shoots produced roots on 16.1 I¼M NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 I¼M BA and 50 g/l sucrose.

In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants