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Candida spp. infections in animals are becoming problematic and very frequent in the last decades. Antimycotic treatment is usually successful, but recently common cases of resistance are recorded. Minimum inhibitory concentration (MIC) test strips is a quantitative assay for determining the MIC of antimicrobial agents against microorganisms to indicate appropriate patient treatment and for identifying resistance patterns.The aim of this paper was the susceptibility testing of Candida strains isolated from animals in the area of Cluj-Napoca to ketoconazole, using MIC strip technique.Materials and methods: A total number of twenty-three Candida spp. strains isolated from both humans and animals were suspended in saline, and 1 ml of the resulted suspension (0.5 McFarland scale) was used to flood the Sabouraud Dextrose agar Petri dish. The suspension was then removed and the strip was placed in the middle of the plate. The incubation was performed at 28Â°C for 48 hours. When the strip is applied onto an inoculated agar surface, the preformed exponential gradient of antimicrobial agent is transferred into the agar matrix.The MIC is read directly from the scale in terms of I¼g/ml, at the point where the edge of the inhibition ellipse intersects with the MIC Test Strip. MIC for the 23 Candida spp. tested strains varied between 0.002 and 8 I¼g/ml, with an average of 3.6 I¼g/ml.Susceptibility testing of 23 Candida spp. isolates from Cluj-Napoca area to ketoconazole using the MIC strips is an accurate, simple, fast and accessible quantitative method, recommend for diagnostic in microbiological laboratories. Â

Susceptibilty Testing of Candida spp. Strains to Ketoconazole Using Minimum Inhibitory Concentration Strip Test