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The aim of this study was to develop and evaluate some validation parameters of a GC-MS sensitive method for the separation, identification, and simultaneous quantification of 18 cis/trans isomers of oleic (FAME C18:1), linoleic (FAME C18:2), and linolenic acid (FAME C18:3) methyl esters. Linearity, sensitivity, and recovery of the developed method were evaluated on calibration solutions. Correlation coefficients (R) were higher than 0.99 in the linear domain for each isomer, the sensitivity of the method was characterized by LOD = 0.03 0.05 g/100 g of fat, and LOQ = 0.09 0.17 g/100 g of fat, and the recovery ranged between 101.32 102.59%. Samples of potato chips were used to demonstrate the applicability of the method and the trans fatty acids (TFA) levels in analyzed sample were below 0.60 g/100 g fat, complying with international recommendations of less than 2 g/100 g of total fat. The proposed GC-MS method allows simultaneous determination of 18 cis/trans isomers (13 trans and 5 cis) of FAME C18 from potato chips.

Development of a Performance Method for Determination of Cis/Trans Isomers of Oleic, Linoleic and Linolenic Acids from Potato Chips by GC-MS