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Temperature is often pointed out as the major factor in determining the bacterial community diversity and has a marked influence on survival and persistence of rhizobial stains in soils. Rhizobial strains obtained from different red clover and alfalfa varieties were examined for their ability to survive under different temperature levels on two different media. Rhizobial isolates either increased or remained constant as estimated by plate count at 20 and 35°C. Significant reduction was detected at 4, 10, 15 and 42°C in the numbers of CFU for both strains analysed. The differences between the two strains showed that Sinorhizobium meliloti survives better than Rhizobium trifolii to extreme temperatures.

The Effect of Temperature Stress on Rhizobium trifolii and Sinorhizobium meliloti Strains In Vitro