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Objective: To establish a rapid, accurate and reliable analytical method for the simultaneous determination of four major anthraquinones in Polygoni Multiflori Radix (PMR) using single reference standard. Methods: The four components including emodin-8-O-β-D(EMG), physcion-8-O-β-D-glucoside, emodin and physcion were separated on an ODS C18 column within 13 min and detected at 280 nm. Emodin was selected as the reference standard, and the response factor for each analyte with respect to emodin were calculated. Robustness were also tested including different columns, equipments, temperatures, detection wavelengths, and other chromatographic conditions which might influence stability of response factors. Results: The method was validated in terms of linearity (r > 0.9995), LOQs (0.820–3.05 ng/mL), LODs (0.180–0.920 ng/mL), precision, accuracy (95.8–103.6%, RSD < 2.80%) and stability. A total of 40 batches of PMR were analyzed and the results were found to have no statistically significant differences compared with those obtained using the external standard method. Conclusion: This work provided a single standard to determine multi-components method for quantitation of four anthraquinones in PMR, which could be applied in the quality control of this herbal drug.

world journal of traditional chinese medicine

Simultaneous Determination of Four Anthraquinones in Polygoni Multiflori Radix with Single Reference Standard by High Performance Liquid Chromatography