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an important role in the modern epidemiological investigations of tuberculosis in animals at the regional and international level. Bovine tuberculosis represents a significant economic burden to the agriculture of the affected countries. From 2000 to 2015 the disease shows cyclicity in private farms in different regions of Bulgaria. This study is a first molecular investigation of animal tuberculosis in the veterinary medicine in country. The macroscopic and microscopic observation of 35 diagnostic materials from slaughtered cattle, received in the National Reference Laboratory of animal tuberculosis were studied with the three molecular methods: RD4PCR, spoligotyping and MIRU-VNTR. In 27 of the examined lymph nodes we found specific lesions for bovine tuberculosis. The findings were confirmed bacteriologically and by conventional PCR. To differentiate M. bovis from other M. tuberculosis complex subtypes, we used primers f lanking specific deletion (RD4) in the genome of M. bovis and obtained the 446 bp DNA pro duct. The spoligotyping subdivided the strains into 3 spoligotypes shared by two to 20 strains. Further molecular investigations of M. bovis strains are needed to characte rize the genetic diversity and population structure of M. bovis strains isolated from cattle in Bulgaria. New information will be added to the global database in the field of molecular epidemiology of the prevalence of M. bovis strains in the cattle population in Bulgaria, which will allow comparative analysis with data from the Balkan region and Europe. Acknowledgements: This work was supported by the grant DN16/12 of the National Science Fund, Bulgaria.

INTERNATIONAL VALIDATION OF ANALYSIS PIPELINES FOR WHOLE GENOME SEQUENCING DATA OF MYCOBACTERIUM TUBERCULOSIS ISOLATES