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Rotavirus infection is an important health problem all over the world. In Russia, under the conditions of the beginning of vaccination against this infection, knowledge about its pathogen is limited by the characteristic with the binary classification (G[P]-genotypes), based on the properties of the VP4 and VP7 genes encoding the rotavirus outer capsid proteins. Information about the other gene segments genotypes, as well as unusual and reassortant strains is not sufficient. The aim of this study was to determine the I (VP6) and E (NSP4) genotypes of rotaviruses detected in Nizhny Novgorod using the multiplex PCR method. We used 55 rotavirus-positive fecal samples from child ren hospitalized with acute intestinal infection from January to May 2018. RNA of rotaviruses was extracted using “RIBO-prep” reagent kit (AmpliSens, Russia). RT-PCR was carried out with reagents manufactured by “Sileks” (Germany). Gand P-genotypes of rotaviruses were determined using previously published primers. To identify Iand E genotypes in multiplex PCR, fragments of 195 bp (I3), 273 bp (I1), 368 bp (I2) and 233 bp (E3), 305 bp (E2), 443 bp (E1), respectively, were amplified and detected by agarose gel electrophoresis. Iand E-genotypes were determined in 51 samples (92.8%). In one sample only E-genotype (1.8%) was revealed, and in three — only I-genotype (5.4%). Mostly, the genotypes were detected in combination I1-E1 (52.7%). The set of I2-E2 was found in 30.9% of cases. In addition, the genotype I1-E2 (5.6%) was identified in three samp les, I2-E1 and I3-E3 (3.6% together) were shown to be sporadic. The following combinations of G-, P-, I-, and E-genotypes were determined: G1-P[8]-I1-E1 (9.1%), G4-P[8]-I1-E1 (7.3%), G9-P[8]-I1-E1 (32.7%), G4-P[8]-I1-E2 (5.5%), G3P[x]-I2-E2 (1.8%), G2-P[4]-I2-E2 (29.1%), G2-P[4]-I2-E1 (1.8%), G2-P[4]-I2-Ex (3.6%), G9-P[8]-I1-Ex (1.8%), GxP[8]-I1-E1 (5.5%), and Gx-P[x]-I3-E3 (1.8%). Thus, the new method to identify the Iand E-genotypes was tested and their distribution was determined. Various combinations of G-, P-, I-, and E-genotypes of rotaviruses have been shown. The genotype G9-P[8]-I1-E1 was predominant (32.7%). The G4-P[8]-I1-E2, G3-P[x]-I2-E2, G2-P[4]-I2-E1 strains had probably a reassortant origin. The study was funded by RFBR (research project No. 18-3400586).

DISTRIBUTION OF ROTAVIRUS G-, P-, I-, AND E-GENOTYPES IN NIZHNY NOVGOROD, RUSSIA