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(57°C and 55°C) and the concentration of magnesium ions in the reaction mixes (2.5 mM) were determined. The PCR conditions and primers specificity were tested using a sample of 16 previously characterized specimens. The results of genotyping by the developed methods fully corresponded to the data of sequencing. Thus, these methods can be used for routine determination of Iand E-genotypes in rotavirus-positive samples and for identification of reassortant rotavirus strains. The study was funded by RFBR, research project No. 18-3400586.

infekciâ i immunitet

CXCL10 GENE PROMOTER POLYMORPHISM A-1447G MODULATES PROTEIN EXPRESSION IN SERUM AND ASSOCIATED WITH INVASIVE ASPERGILLOSIS IN FEMALE ONCOHEMATOLOGICAL PATIENTS