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Received: May 13, 2018; Accepted: May 22, 2018; Published: May 25, 2018 Today generation of single-chain fragment variable (scFv) by phage display has become an established technique and could be used to select a completely functional antigen-binding fragment. Detailed overviews about this in vitro selection technology are given in different reviews [1-3]. A scFv is a molecule of about ~30 kDa and consists of the variable heavy (VH) and variable light (VL) chain joined together by a flexible peptide linker of about 15 amino acids. It is half the size of the antigen-binding (Fab) fragment and retains the specificity of the parent immunoglobulin.

Single-chain fragment variable (scFv) with medical potential