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Sleeping Beauty (SB) exhibits efficient cut-and-paste transposition in a wide range of mammalian cells. Using this characteristic, a new vector system (the SB system) was previously developed for integration of target genes into the host genome and is now used as a research tool and for gene therapy. However, there are concerns about the retransposition and the size restriction to be integrated into this system. To overcome these issues, we constructed a modified transposon vector system with lox sequences and estimated its transposable activity and Cre/lox recombination ability in HeLa cells. We also estimated the possibility of the retransposition by plasmids with defective inverted repeat/direct repeat structures presumed to be taken after Cre/lox recombination. We demonstrated that our modified transposon vector system could efficiently cause recombination mediated by the Cre/lox system following the transposition of the target genes into the genome and eliminate the possibility of retransposition of the genes. We believe our modified transposon vector system could be used not only as a research tool but also as a safer and more versatile gene integration system in the medical field. *Correspondence to: Kazuyoshi Kaminaka, Development Department, Research and Development Division, Kikuchi Research Center, KM Biologics Co., Ltd., 1314-1 Kyokushi Kawabe, Kikuchi-city, Kumamoto 869-1298, Japan, Tel: +81968-37-4064; Fax: +81-968-37-3108; E-mail: kaminaka@kmbiologics.com

A Modified Sleeping Beauty Transposon Vector System Integrates and Settles Genes in the Genome