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An abstract of the thesis of Keith Forrest Dockery for the Master of Science in Biology presented November 18, 1994. Title: Investigations on Glycolipid Production by Pseudomonas putida Grown on Toluene in Batch and Continuous Culture Conditions Utilization of toluene by Pseudomonas putida as its sole carbon and energy source affects morphology, outer membrane protein composition, and glycolipid production. Two strains of P. putida were found to utilize toluene and to coexist in continuous and batch culture. The two strains were designated translucent and opaque, based upon their readily identifiable coloration when grown on Luria agar. The translucent strain was the dominant strain in continuous culture conditions. The outer membrane proteins of P. putida were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. When toluene is the carbon and energy source, the trend in protein composition was towards a general increase in concentration of lower molecular weight proteins ( <70Xl04 mol wt). A similar decrease occurred in the concentration of higher molecular weight proteins in the range of 70X104-9X104 mol wt. P. putida produces

Investigations on Glycolipid Production by Pseudomonas Putida grown on Toluene in Batch and Continuous Culture Conditions