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i Dedication iii Acknowledgements iv List of Tables vii List of Figures viii List of Abbreviations x Section 1: Scope of Thesis 1 Section 2: Background 3 2.1 Existing Primary Water Testing Techniques: Membrane Filtration and DST 7 2.2 Conceptual Alternative Testing Methods for the Developing World 9 2.3 Fluorescence Theory 11 2.4 Current State of Research 17 2.5 Possible Mechanisms for Bacterial Tryptophan Fluorescence 19 2.6 Extensibility of Fluorescent in-situ Sensors 21 Section 3: Experimental Methods & Results 25 3.1 Sample Collection 25 3.2 Membrane Filtration Procedure 27 3.3 SMF4 Fluorimeter Procedure 30

Assessing a Fluorescence Spectroscopy Method for In-Situ Microbial Drinking Water Quality