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A new, stable and accurate high performance liquid chromatography ultraviolet spectrophotometric method for quantitation of Ciprofloxacin in human plasma was developed and validated. The chromatographic separation was performed on a reversed phase BDS (base deactivated silica) C18 analytical column; 150 × 4.6; 5 μm particle size. The mobile phase consisted of a mixture of 1 ml triethylamine (TEA)/1L, water and acetonitrile (80:20 v/v). The pH of mobile phase was adjusted to 2.5 by using phosphoric acid and the detector was set at a wavelength of 280 nm. Accuracy, precision, linearity and stability of the analytical method were done. The regression equations are linear (R 2 was 0.999) over the calibration curve concentration range of (0.10-8.0) µg/ml. Inter and Intra precision (% CV) were less than 6.102% and 6.82% respectively.

Relative Bioavailability of Ciprofloxacin Doses (750 and 1000) mg in Healthy Male Volunteers by Using HPLC Method