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The articular cartilage overlying the bone consists of a network of collagen fibres. This network is essential to cartilage integrity, usually suffering damage in degenerative joint diseases such as osteoarthritis. We have been applying a number of techniques to study the bone-cartilage interface and of changes occurring in this with disease. The bone-cartilage samples with disease were investigated for their structural, elemental and molecular properties. The bone-cartilage samples with disease were characterized by scanning electron microscopyenergy dispersive X-ray spectroscopy (SEM-EDX) and Fourier Transform infrared spectroscopy (FTIR) and Fourier transform (FT) Raman spectroscopy. The energy dispersive X-ray (EDX) analysis confirmed nearly stoichiometric samples. The Raman spectra made it possible to monitor the changes in the main bone constituents: the mineral component with the apatite band at 960 cm−1, the organic component with the collagen amide III band at 1268cm−1.Present results have been obtained on sections of bone not displaying evidence of an osteoarthritic lesion and can be used as a baseline against which diseased bone can be compared.

Structural, Elemental and Molecular Characterization of Normal And Osteoarthritic Human Articular Cartilage