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The examination of saliva traces left on cigarette butts as evidences are complicated due to the availability of biological material in trace amounts and its rapid degradation due to extreme effects of environmental factors. This study is aimed to assess the DNA purity and quantify the amount of DNA preserved in saliva found on cigarette butts subjected to various temperatures and humidity. Isolation of cell material from biological traces on forensic evidence is often a serious challenge to solving forensic cases. Successful isolation of high-grade DNA from cell material even in critically low quantity could be achieved in examination of traces. The proper collecting and storage of the material is very important for successful DNA typing from saliva traces and epithelial cells from the lips and oral cavity. Meeting these conditions would increase the chances for successful DNA profiling of biological traces on evidence of an earlier date. In the presented forensic cases the opportunity for solving the crime was given by the vices of the suspects. In this case one cigarette butt found at scene of crime was the only evidence to detect the accused. DNA is extracted from salivary epithelial cells adheres to the cigarette butt and profiled successfully. DNA profiles of suspected accused and the cigarette butt are matched in the source.

“Only Cigarette Butt is Left, DNA Fingerprinting Traps the Theft”