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Research on the stem cell biology of the minipig is rapidly developing. Although research on mouse and human stem cells currently predominates over that in other species, data from these species have provided a good foundation for current and future porcine stem cell research. In addition, the increasing popularity of alternate-species models in the study of human diseases and disease mechanisms has spurred porcine stem cell research. As a source of pluripotent embryonic stem cells, the pig presents several challenges as compared with mice and humans. Nonetheless, porcine minipig embryonic germ cells have been recently produced and may prove particularly useful for in vitro and in vivo differentiation studies, in gene targeting, and in the creation of transgenic animals. Regarding research on somatic stem cells, transplantation studies of mesenchymal stem cells into porcine heart, cartilage, and bone have yielded very promising results. The microminipig is a small minipig that was established by Fuji Micra Inc. Here, we investigated whether induced pluripotent stem cells (iPSCs) could be derived from somatic cells of the microminipig by using Sendai viruses to introduce human pluripotency transcription factors. Although virally transduced fibroblasts derived from the microminipig generated ES-like colonies, when the cells were introduced into SCID mice, we could not detect the formation of teratomas containing ectodermal, mesodermal, and endodermal tissues. These findings suggest that further research is required to identify the signaling pathways and supplementary factors required for the induction of pluripotent microminipig iPSCs.

journal of veterinary science and animal husbandry

Derivation of Induced Pluripotent Stem (iPS)-like Cells from Microminipig Somatic Cells by Sendai Viral Transduction of 4 HumanG, OCT4, SOX2, KLF4, and c-MYC