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K. LOGANATHASAMY Department of Veterinary Biochemistry, Madras Veterinary College, CHENNAI (T. N.) INDIA Email: loganathasamy@tanuvas. org.in Abstract : The present investigation has been undertaken to study the effects of exogenous supplementation of sodium nitroprusside (SNP), a nitric oxide (NO) donor on in vitro sperm characteristics of buffalo semen. Buffalo straws from 6 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-51. The frozen straws were thawed at 37°C for 30 seconds and emptied into 15 ml sterile plastic centrifuge tube containing 1 ml of capacitation medium (control), addition of 100 μM/ml of SNP (SNP treatment I) and 100 nM/ml of SNP (SNP treatment II) and were incubated at 37°C for 1 hour. After 1 hour incubation, the progressive motility was studied under bright field microscopy. Sperm motility was significantly (P<0.01) lowered in SNP treatment I (11.67% ± 1.67) and II (23.33% ± 2.11) as compared to control (43.33% ± 2.11). Between treatments, sperm motility was significantly (P<0.01) high in SNP treatment II than SNP treatment I. The sperm viability was assessed by the supravital Eosin and Nigrosin stain method. Significantly (P<0.01) higher percentage of spermatozoa were alive in control (56.67% ± 0.88) in comparison with SNP treatment I (25.08% ± 1.19) and II (37.83% ± 1.27). But,more spermatozoa were significantly (P<0.01) alive in SNP treatment II than SNP treatment I. The sperm morphology was determined by Rose Bengal stain technique. Morphologically normal spermatozoa were significantly (P<0.01) more in control (83.00% ± 0.62) when compared to SNP treatment I (53.00% ± 0.59) and II (68.42% ± 0.87). In similar way, significantly (P<0.01) higher proportions of spermatozoa were morphologically normal in SNP treatment II than SNP treatment I. From this study, it is concluded that addition of SNP, a NO donor has detrimental effects on the sperm motility, viability and morphology of frozen thawed buffalo semen on concentration dependant manner.

Influence of sodium nitroprusside on sperm motility, viability and morphology of frozen thawed buffalo semen