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Extremophilic bacteria surviving in extreme environmental conditions possess the special abilities to adapt adverse environment. There are very high salinization and alkalinization contents in Panjin Red Beach’s soil. A productive alkline protease strain HHT597 was isolated from the soil samples and identified as Bacillus altitudinis based on the 16s rDNA sequence. The HHT597 strain was characterized as a gram positive, rod-shaped bacterial strain with flagellum. The optimum temperature for protease activity by the strain HHT597 was 60°C. There was a broad pH active range of protease from 7.0–11.0 with having optima pH 9.0. The media containing 2.0% glucose and 1% yeast extract as carbon and nitrogen sources could improve protease activity. The strain HHT597 could degrade casein, hemoglobin (HGB) and albumin (ALB) protein. The results indicated that the strain HHT597 might be potential for biotechnological applications.

IDENTIFICATION OF AN ALKALINE PROTEASE PRODUCING BACTERIUM ISOLATED FROM PANJIN RED BEACH, CHINA