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The extraction of methyl esters of tuna oil has been performed, and the extraction behavior is discussed. The effects of AgNO3concentration, the addition of water-soluble polar solvents such as methanol to aqueous silver nitrate solutions and the presence of organic solvent on the recovery yield of PUFA-ME have been also investigated. Results show that docosahexaenoic acid methyl ester (DHA-ME) and eicosapetaenoic acid methyl ester (EPA-ME)  and can be selectively extracted into silver nitrate solutions.

Polyunsaturated fatty acid enrichment by complexation with silver ion