English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Aeromonas spp. use T3SS to secrete and transport effector proteins to the host cells. These proteins play a major role in bacteria virulence by interfering with the signaling cascades and by disrupting the cytoskeleton structure of the host cell. Despite tremendous efforts, structural and functional information regarding AcrV tip protein of T3SS remains elusive. In this study, we cloned the gene encoding the AcrV protein from Aeromonas hydrophila AH-1 and inserted it into the pET-M expression vector. The pET-M vector containing AcrV gene was transformed and expressed in E.coli BL21 (DE3) cells. The recombinant AcrV protein was purified by affinity chromatography using Ni-NTA column. The obtained AcrV with high purity can be used for structural and functional studies.  

Cloning, expression, and purification of Acrv tip protein from Aeromonas hydrophila using Escherichia coli host cells