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Silver nanoparticles (SNPs) are one of metallic nan op rticles widely applied in many fields. Research of SNPs application in plant tissue cultur e has been gaining attention in recent years. Moreover, novel plant tissue culture systems have b een researched and developed for improving SNPs uptake capability in medium. In this study, we investigated effects of SNPs on Chrysanthemum morifolium ramat. cv. "JIMBA" growth and its ability in 3 cult re systems: in vitro solid medium system, in vitro liquid medium system and microponic (combined of micropropagation and hydroponic). The 3 cm Chrysanthemum shoots and silver nanoparticles of diameter smaller than 20 nm were used in the experi ments. After 4 weeks, the results showed that the SNPs concentration was suitable for growth of Chrysanthemum in vitro solid medium system, in vitro liquid medium system and microponic was 1.5, 1.5 a nd 5 ppm, respectively. Microponic system not only improved plant growth bu t also reduced the succulent phenomenon. SNP uptake was likely dependent on concentrations a nd culture systems. At low concentrations (1, 5, and 10 ppm), SNPs were completely absorbed a ft r 4 weeks of culture in all systems, but not at high concentrations (20 ppm). The amount of absorbed silver nanoparticles was directly proportional to the culture period and inversely pr oportional to the concentration of SNPs supplemented to the medium. In all of our investiga ted systems, the hydroponic system showed the highest capability of SNPs absorption.

THE EFFECTS OF SILVER NANOPARTICLES ON GROWTH OF Chrysanthemum morifolium Ramat. cv. "JIMBA" IN DIFFERENT CULTURAL SYSTEMS